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Rat TIMP-1 ELISA Kit

Rat TIMP-1 ELISA Kit

ArtNr	ELR-TIMP1-5
Hersteller	Raybiotech
Menge	5 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit 1 Plate Kit 2 Plate Kit 5 Plate Kit
Kategorie	Oncology Neuroscience Neural Signaling ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Bladder Cancer
Typ	Elisa-Kit
Specific against	Rat (Rattus norvegicus)
Host	Hamster - Armenian
Sensitivity	4 pg/ml
Citations	1. Omi H., et al. Low-intensity pulsed ultrasound stimulation enhances TIMP-1 in nucleus pulposus cells and MCP-1 in macrophages in the rat. J Orthop Res. 2008 Jun,26(6):865-71. doi: 10.1002/jor.20545. 2. Lijnen P, Papparella I, Petrov V, Semplicini A, Fagard R. Angiotensin II-stimulated collagen production in cardiac fibroblasts is mediatd by reactive oxygen species. J Hypertens. 2006,24:757–766. 3. Rohrbach S., et al. Enhanced myocardial vitamin C accumulation in left ventricular hypertrophy in rats is not attenuated with transition to heart failure. European Journal of Heart Failure 10 (2008) 226–232, doi:10.1016/j.ejheart.2008.01.001
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Similar products	TIMP-1
Versandbedingung	Gekühlt
Lieferbar
Specificity	This ELISA kit shows no cross-reactivity with any of the cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, LIX, Leptin, MCP-1, MIP-3 alpha, beta- NGF, TNF-alpha, VEGF.
Manufacturer - Category	ELISA Kits\|Sandwich ELISA
Manufacturer - Targets	TIMP-1
Shipping Temperature	Blue ice
Storage Conditions	?-20°C
Description	RayBio® Rat TIMP-1 ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples. Lead time: Typically ships within 1-2 business days. No Friday shipments.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum
Design Principle	Sandwich-based
Method of Detection	Colorimetric
Solid Support	96-well Microplate
Kit Components	Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Other Materials Required	Distilled or deionized water Precision pipettes to deliver 2 ul to 1 ul volumes Adjustable 1-25 ul pipettes for reagent preparation 100 ul and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline	Prepare all reagents, samples and standards as instructed in the manual. Add 100 ul of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4C. Add 100 ul of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 ul of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 ul of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 ul of Stop Solution to each well. Read at 450 nm immediately.
Product Features	Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Result Output	Quantitative
UNSPSC Code	41116158
Manufacturer - Specificity	This ELISA kit shows no cross-reactivity with any of the cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, LIX, Leptin, MCP-1, MIP-3 alpha, beta- NGF, TNF-alpha, VEGF.
Recommended Dilution	100 fold

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

ELR-TIMP1-5.pdf

ELR-TIMP1-5-datasheet.pdf

ELR-TIMP1-5-safety-datasheet.pdf