Rat TNF-alpha ELISA

Hersteller Raybiotech
Kategorie
Typ Elisa-Kit
Specific against Rat
Menge 1 x 96-Well Strip Microplate Kit
Host E.coli
ArtNr ELR-TNFa-1
Eclass 6.1 32160605
Eclass 9.0 32160605
Lieferbar
Quantity options
Short description
RayBio Rat TNF-alpha ELISA Kit for serum, plasma, and cell culture supernatants
Target Name and Synonyms
TNF alpha
Solid Support
96-well Microplate
Design Principle
Sandwich-based
Result Output
Quantitative
Method of Detection
Colorimetric
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum
Sensitivity
25 pg/ml
Detection Range
25 pg/ml - 20000 pg/ml
Recommended Dilution (serum & plasma)
2 fold
Specificity
This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1alpha, IL- 1beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3alpha, beta- NGF, TIMP-1, VEGF).
Product Features
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 ul to 1 ul volumes
  • Adjustable 1-25 ul pipettes for reagent preparation
  • 100 ul and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Summary
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 ul of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4C.
  4. Add 100 ul of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 ul of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 ul of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 ul of Stop Solution to each well.
  11. Read at 450 nm immediately.
Storage
May be stored for up to 6 months at 2C to 8C from the date of shipment. Standard (recombinant protein) should be stored at -20C or -80C (recommended at -80C) after reconstitution. Opened Microplate Wells or reagents may be store for up to 1 month at 2C to 8C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20C.
Citation
1. Breikaa, Randa M., Ashraf B. Abdel-Naim, and Hisham A. Mosli. "Adverse Testicular Effects of Botox in Mature Rats." Toxicology and Applied Pharmacology. ScienceDirect, 14 Jan. 2014. Web.
2. Peng H, Zhi-Fen W, Su-Mei J, et al. Blocking abdominal lymphatic flow attenuates acute hemorrhagic necrotizing pancreatitis -associated lung injury in rats. J Inflamm (Lond). 2013, 10(1):9.
3. Birt JA, Nabli H, Stilley JA, et al. Elevated Peritoneal Fluid TNF-alpha Incites Ovarian Early Growth Response Factor 1 Expression and Downstream Protease Mediators: A Correlation With Ovulatory Dysfunction in Endometriosis. Reprod Sci. 2013 May, 20(5):514–523.
4. Gandhi S, Srinivasan B, Akarte AS. Aliskiren improves insulin resistance and ameliorates diabetic renal vascular complications in STZ-induced diabetic rats. J Renin Angiotensin Aldosterone Syst. 2013, 14(1):3–13.
5. Awaad AS, El-Meligy RM, Al-Jaber NA, et al. Anti-Ulcerative Colitis Activity of Compounds from Euphorbia granuleta Forssk. Phytother Res. 2013 Apr 11.
6. Gullaiya S, Nagar A, Dubey V, et al. Modulation of disease related immune events by demethoxycurcumin against autoimmune arthritis in rats. Biomed Aging Pathol. 2013 Jan, 3(1):7–13.
7. Zaafan MA, Zaki HF, El-Brairy AI, Kenawy SA. Protective effects of atorvastatin and quercetin on isoprenaline-induced myocardial infarction in rats. Bulletin of Faculty of Pharmacy, Cairo University. 2013 Jun, 51(1):35–41.
8. Abdelwahab SI. Protective mechanism of gallic acid and its novel derivative against ethanol-induced gastric ulcerogenesis: Involvement of immunomodulation markers, Hsp70 and Bcl-2-associated X protein. Int Immunopharmacol. 2013, 16(2):296–305.
9. Mohamed SH, Ahmed HH, Farrag ARH, et al. Cynara scolymus for relieving on nonalcoholic steatohapatitis induced in rats.Int J Pharm Pharmaceut Sci. 2013, 5(Suppl. 1):57–66.
10. Oyewo EB, Adewale A, Abdulfatai Ayoade A, Akanji MA. Repeated Oral Administration of Aqueous Leaf Extract of Moringa oleifera modulated immunoactivities in Wistar Rats. J Nat Sci Res. 2013, 3(6):100–109.
11. Mansour A, Mohamed B, Aboul-Fotouh S, et al. Effects of pentoxifylline, 7-nitroindazole, and imipramine on tumor necrosis factor-&, alpha, and indoleamine 2, 3-dioxygenase enzyme activity in the hippocampus and frontal cortex of chronic mild-stress-exposed rats. Neuropsych Dis Treat. 2013, 9:697–708.
12. Peng H, Zhi-Fen W, Su-Mei J, et al. Blocking abdominal lymphatic flow attenuates acute hemorrhagic necrotizing pancreatitis -associated lung injury in rats. J Inflamm (Lond). 2013, 10(1):9.
13. Liu A., Pu Y., Zheng Y., Cai H., Bin Y. Therapeutic efficacies of chitosan against Pneumocystis pneumonia of immunosuppressed rat. Parasite Immunology doi: 10.1111/pim.12117 [Epub Ahead of Print]
14. Seif A., Abdelwahed D. Vitamin D ameliorates hepatic ischemic/reperfusion injury in rats. Journal of Physiology and Biochemistry, April 22, 2014 doi.10.1007/s13105-014-0335-2 [Epub Ahead of Print]
15. Zhang Y., Zhang S., Cui N. Intravenous infusion of mesenteric lymph from severe intraperitoneal infection rats causes lung injury in healthy rats. World J Gastroenterol 2014 April 28, 20(16): 4771-4777
16. Mohammed D., Elmelegy A., El-Azaz L., Kawy H., AbdElsamad A., El-Kharashi O., Hepatoprotective effects of early pentoxifylline administration on hepatic injury induced by concanavalin A in rat. Canadian Journal of Physiology and Pharmacology, 10.1139/cjpp-2014-0085 [Epub Ahead of Print]
17. El-Naga R., Ahmed H., Haleen E. Effects of indole-3-carbinol on clonidine-induced neurotoxicity in rats: Impact on oxidative stress, inflammation, apoptosis and monoamine levels. Neurotoxicology 2014. Epub ahead of print
18. Sun Peng., Zhou K., Wang S., Li P., Chen S., et al. Involvement of MAPK/NF-kappaB Signaling in the Activation of the Cholinergic Anti-Inflammatory Pathway in Experimental Colitis by Chronic Vagus Nerve Stimulation. PLOS One Published: August 02, 2013 DOI: 10.1371/journal.pone.0069424
19. Helmy M., et al. ROLE OF NITRERGIC AND ENDOTHELIN PATHWAYS MODULATIONS IN CISPLATIN-INDUCED NEPHROTOXICITY IN MALE RATS. J Physiol Pharmacol. 2014 Jun, 65(3):393-9.
20. El-Bakly W., Hasanin., Hypericum Perforatum Decreased Hippocampus TNF-alpha and Corticosterone Levels with No Effect on Kynurenine/Tryptophan Ratio in Bilateral Ovariectomized Rats. Korean J Physiol Pharmacol. 2014 Jun, 18(3):233-239.
21. El-Bakly W., Hasanin., Hypericum Perforatum Decreased Hippocampus TNF-alpha and Corticosterone Levels with No Effect on Kynurenine/Tryptophan Ratio in Bilateral Ovariectomized Rats. Korean J Physiol Pharmacol. 2014 Jun, 18(3):233-239.
22. Asthana G., et al. Mannosylated Chitosan Nanoparticles for Delivery of Antisense Oligonucleotides for Macrophage Targeting. Biomed Res International. Volume 2014, Article ID 526391, 17 pages http://dx.doi.org/10.1155/2014/526391
23. Ahmed M., et al. Pomegranate extract protects against cerebral ischemia/reperfusion injury and preserves brain DNA integrity in rats. Life Sciences.Available July 2014. http://dx.doi.org/10.1016/j.lfs.2014.06.023
24. Kumar A., et al. Effect of Licofelone—A Dual COX/5-LOX Inhibitor in Intracerebroventricular Streptozotocin-Induced Behavioral and Biochemical Abnormalities in Rats. Journal of Molecular Neuroscience. Sept 2014, epub ahead of print. DOI 10.1007/s12031-014-0414-4
25. Saleh DO., et al. Thioacetamide-Induced Liver injury: Protective Role of Genistein. Canadian Journal of Physiology and Pharmacology, 10.1139/cjpp-2014-0192
26. Srivastava M., et al. Nanoemulgel (NEG) of Ketoprofen with eugenol as oil phase for the treatment of ligature-induced experimental periodontitis in Wistar rats. Drug Delivery Sept 2014, epub ahead of print.
27. Aral C., et al. Alveolar Bone Protective and Hypoglycemic Effects of Systemic Propolis Treatment in Experimental Periodontitis and Diabetes Mellitus. Journal of Medicinal Food, Sept 2014, epub ahead of print. DOI: 10.1089/jmf.2013.3137
28. Abdel-Reheim E., et al. Fatty-Sucrosed Diet/Minimal Dose of Streptozotocin Treated Rat: A Novel Model of Gestational Diabetes Mellitus, Metabolic and Inflammatory Insight. J Diabetes Metab 2014, 5:9 http://dx.doi.org/10.4172/2155-6156.1000430
29. Gandhi S., et al. Aliskiren improves insulin resistance and ameliorates diabetic renal vascular complications in STZ-induced diabetic rats. Journal of the Renin-Angiotensin-Aldosterone System. 14(1) 3–13. DOI: 10.1177/1470320312452766

Arbeitsanleitung

ELR-TNFa-1.pdf

Menge: 1 x 96-Well Strip Microplate Kit
Lieferbar: In stock
Listenpreis: 482,90 €
Preis: 482,90 €
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