Recombinant Human CNTF

Hersteller Raybiotech
Kategorie
Typ Proteins
Specific against Human
Menge 20 ug
ArtNr 229-20182
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Short Description
Purified recombinant CTNF expressed in E. Coli
Description
This product is a 199 amino acid, Escherichia coli-derived recombinant protein corresponding to mature human ciliary neutotrophic factor (CTNF), a survival factor for various neuronal cell types believed to prevent the degeneration of motor axons after axotomy.
Protein Name
CNTF
Molecular Weight (kDa)
22.7kD
Purity
>98% by SDS PAGE/HPLC analysis
Concentration
Total protein concentration ca.0.1mg/ml after reconstitution
Preservative
None present.
Endotoxin Level
<1EU/ug
Buffers
Phosphate buffered saline
Protein Type
Recombinant Protein
Recommended Applications
ELISA (0.2-0.4ng/well), Functional Assays (50-150ng/ml), Western Blotting (1.5-3.0ng/lane)
Biological activity
The ED50 as determined by the dose-dependent proliferation of human TF-1 cells is 50-150ng/ml.
Reconstitution
Reconstitute with 0.2ml of 5mM sodium phosphate, pH 7.5.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommends that the vial is gently mixed after reconstitution. For extended storage, the addition of 0.1% bovine serum albumin (BSA) is recommended.
Storage/stability
Prior to reconstitution store at +4° C.
After reconstitution store at -20° C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. This protein may be stored for3 months from date of reconstitution.
Menge: 20 ug
Lieferbar: In stock
Listenpreis: 528,00 €
Preis: 528,00 €
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