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Recombinant Human CNTF

Recombinant Human CNTF

Manufacturer	Raybiotech
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins
Specific against	Human
Amount	20 ug
Item no.	229-20182
eClass 6.1	34160400
eClass 9.0	42020190
Available
Short Description	Purified recombinant CTNF expressed in E. Coli
Description	This product is a 199 amino acid, Escherichia coli-derived recombinant protein corresponding to mature human ciliary neutotrophic factor (CTNF), a survival factor for various neuronal cell types believed to prevent the degeneration of motor axons after axotomy.
Protein Name	CNTF
Molecular Weight (kDa)	22.7kD
Purity	>98% by SDS PAGE/HPLC analysis
Concentration	Total protein concentration ca.0.1mg/ml after reconstitution
Preservative	None present.
Endotoxin Level	<1EU/ug
Buffers	Phosphate buffered saline
Protein Type	Recombinant Protein
Recommended Applications	ELISA (0.2-0.4ng/well), Functional Assays (50-150ng/ml), Western Blotting (1.5-3.0ng/lane)
Biological activity	The ED₅₀ as determined by the dose-dependent proliferation of human TF-1 cells is 50-150ng/ml.
Reconstitution	Reconstitute with 0.2ml of 5mM sodium phosphate, pH 7.5. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommends that the vial is gently mixed after reconstitution. For extended storage, the addition of 0.1% bovine serum albumin (BSA) is recommended.
Storage/stability	Prior to reconstitution store at +4° C. After reconstitution store at -20° C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. This protein may be stored for3 months from date of reconstitution.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.