|
Hersteller |
Raybiotech
|
|
Kategorie |
|
|
Typ |
Proteins |
|
Specific against |
Human |
|
Menge |
1 mg |
|
ArtNr |
229-20189 |
|
eClass 6.1 |
34160400 |
|
eClass 9.0 |
42020190 |
|
Lieferbar |
 |
| Short Description |
Purified recombinant leptin expressed in E.coli. Sterile filtered through a 0.2 micron filter. |
| Introduction |
Leptin is a major adipose-derived hormone and member of the leptin family, which plays a key role in appetite control and the regulation of energy expenditure.
Secreted leptin associates with proteins within the bloodstream and is transported to the brain, where it inhibits or up-regulates the activity of neuropeptides such as neuropeptide Y (NPY) and alpha-melanocyte-stimulating hormone respectively. Leptin is a recognised marker of obesity, with a correlation between circulating levels of leptin and body fat mass, and defects in leptin production result in severe obesity. Regulators of leptin expression include insulin, cortisol, cAMP and thiazolidinediones. |
| Protein Name |
LEPTIN |
| Molecular Weight (kDa) |
16.0kD (147 amino acid residues). |
| Purity |
>98% by SDS PAGE and HPLC analysis. |
| Formulation |
Lyophilized with 0.1% TFA |
| Concentration |
ca.1.0 mg/ml after reconstitution |
| Preservative |
None present |
| Endotoxin Level |
<1EU/ug |
| Protein Type |
Recombinant Protein |
| Recommended Applications |
ELISA (0.2-0.4ng/well), Functional Assays, Western Blotting (1.5-3.0ng/lane) |
| Biological activity |
129-20189 is biologically active in the ob/ob mouse obesity model. The ob/ob mice were treated via intraperitoneal injection once daily at a dose of 5 µ g Leptin/gm of body weight for 7 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed to saline-treated controls. |
| Reconstitution |
Reconstitute with 1.0 ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommends that the vial is gently mixed after reconstitution. For extended storage, the addition of 0.1% bovine serum abumin (BSA) is recommended. |
| Storage/stability |
Prior to reconstitution store at +4° C.
After reconstitution store at -20° C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. This protein may be stored for3 months from date of reconstitution. |
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