Recombinant Human LEPTIN

Hersteller Raybiotech
Kategorie
Typ Proteins
Specific against Human
Menge 1 mg
ArtNr 229-20189
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Short Description
Purified recombinant leptin expressed in E.coli. Sterile filtered through a 0.2 micron filter.
Introduction
Leptin is a major adipose-derived hormone and member of the leptin family, which plays a key role in appetite control and the regulation of energy expenditure.

Secreted leptin associates with proteins within the bloodstream and is transported to the brain, where it inhibits or up-regulates the activity of neuropeptides such as neuropeptide Y (NPY) and alpha-melanocyte-stimulating hormone respectively. Leptin is a recognised marker of obesity, with a correlation between circulating levels of leptin and body fat mass, and defects in leptin production result in severe obesity. Regulators of leptin expression include insulin, cortisol, cAMP and thiazolidinediones.
Protein Name
LEPTIN
Molecular Weight (kDa)
16.0kD (147 amino acid residues).
Purity
>98% by SDS PAGE and HPLC analysis.
Formulation
Lyophilized with 0.1% TFA
Concentration
ca.1.0 mg/ml after reconstitution
Preservative
None present
Endotoxin Level
<1EU/ug
Protein Type
Recombinant Protein
Recommended Applications
ELISA (0.2-0.4ng/well), Functional Assays, Western Blotting (1.5-3.0ng/lane)
Biological activity
129-20189 is biologically active in the ob/ob mouse obesity model. The ob/ob mice were treated via intraperitoneal injection once daily at a dose of 5 µ g Leptin/gm of body weight for 7 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed to saline-treated controls.
Reconstitution
Reconstitute with 1.0 ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommends that the vial is gently mixed after reconstitution. For extended storage, the addition of 0.1% bovine serum abumin (BSA) is recommended.
Storage/stability
Prior to reconstitution store at +4° C.
After reconstitution store at -20° C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. This protein may be stored for3 months from date of reconstitution.
Menge: 1 mg
Lieferbar: In stock
Listenpreis: 528,00 €
Preis: 528,00 €
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