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Recombinant Human PAI-1

Recombinant Human PAI-1

Hersteller	Raybiotech
Kategorie	Oncology Peptides & Proteins Recombinant Proteins Cell Biology Cell Status Senescence By Organ Breast Cancer Pancreatic Cancer
Typ	Proteins
Specific against	Human
Menge	10 ug
ArtNr	229-20190
Targets	SERPINE1
eClass 6.1	34160400
eClass 9.0	42020190
Lieferbar
Short Description	Purified recombinant PAI-1 expressed in E.coli.
Introduction	PAI-1 (plasminogen activator inhibitor-1) is a serine protease inhibitor and member of the serpin family, which acts as the primary inhibitor of tissue plasminogen activator (tPA) and urokinase plasminogen activator (uPA), and hence fibrinolysis. Increased levels of PAI-1 are associated with diabetes, cardiovascular disease and an increased occurrence of thrombosis in obesity, and can be induced by obesity related factors including insulin, glucose and VLDL (very-low-density lipoprotein).
Protein Name	PAI-1
Molecular Weight (kDa)	42.7kD (379 amino acid residues).
Purity	>95% by SDS PAGE and HPLC analysis.
Formulation	10mM Sodium Phosphate
Concentration	ca.0.1mg/ml after reconstitution
Preservative	None present
Endotoxin Level	<1EU/ug
Protein Type	Recombinant Protein
Recommended Applications	Functional Assays
Biological activity	Determined by its inhibitory effect against single chain tPA induced cleavage of a chromogenic substrate in Imidazole Buffer at 37° C. Half maximal inhibition against 1.0 ug/ml of single chain tPA was obtained at a concentration of 2.0 ug/ml.
Reconstitution	Reconstitute with 100ul distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommends that the vial is gently mixed after reconstitution. For extended sotrage, the addition of 0.1% BSA is recommended.
Storage/stability	Prior to reconstitution store at +4° C. After reconstitution store at -20° C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. This protein may be stored for3 months from date of reconstitution.

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