|
Hersteller |
Raybiotech
|
|
Kategorie |
|
|
Typ |
Proteins |
|
Specific against |
Human |
|
Menge |
25 ug |
|
ArtNr |
229-20192 |
|
eClass 6.1 |
34160400 |
|
eClass 9.0 |
42020190 |
|
Lieferbar |
 |
| Short Description |
Purified recombinant visfatin expressed in E.coli. |
| Introduction |
Visfatin is a putative cytokine originally identified as PBEF (Pre-B cell-enhancing factor), subsequently determined to be a nicotinamide phosphoribosyltransferase (NAMPT) enzyme and member of the NAPRTase family, involved in catalyzing the biosynthesis of NAD from nicotinamide.
Visfatin is highly expressed in bone marrow, muscle and liver tissue and increases in expression in response to endotoxin, in amniotic epithelial cells following lipopolysaccharide (LPS) stimulation, and during the activation of T and B cells, dendritic cells and macrophages, suggesting a role in inflammatory processes. |
| Protein Name |
VISFATIN |
| Molecular Weight (kDa) |
52.5kD (465 amino acid residues). |
| Purity |
>98% by SDS PAGE and HPLC analysis. |
| Formulation |
10mM HCl |
| Concentration |
Total protein concentration ca.0.1mg/ml after reconstitution. |
| Preservative |
None present |
| Endotoxin Level |
<1EU/ug |
| Protein Type |
Recombinant Protein |
| Recommended Applications |
Functional Assays |
| Biological activity |
The ED 50 was determined by the dose-dependant proliferation of the RPMI 8226 cells. The expected ED 50 for this effect is 15.0-20.0 ng/ml. |
| Reconstitution |
Reconstitute with 0.25ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommends that the vial is gently mixed after reconstitution.
N.B. Due to solubility reasons, it is recommended that the solution be kept at low pH.. |
| Storage/stability |
Prior to reconstitution store at +4° C.
After reconstitution store at -20° C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. This protein may be stored for3 months from date of reconstitution. |
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