Recombinant Human VISFATIN

Hersteller Raybiotech
Kategorie
Typ Proteins
Specific against Human
Menge 25 ug
ArtNr 229-20192
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Short Description
Purified recombinant visfatin expressed in E.coli.
Introduction
Visfatin is a putative cytokine originally identified as PBEF (Pre-B cell-enhancing factor), subsequently determined to be a nicotinamide phosphoribosyltransferase (NAMPT) enzyme and member of the NAPRTase family, involved in catalyzing the biosynthesis of NAD from nicotinamide.

Visfatin is highly expressed in bone marrow, muscle and liver tissue and increases in expression in response to endotoxin, in amniotic epithelial cells following lipopolysaccharide (LPS) stimulation, and during the activation of T and B cells, dendritic cells and macrophages, suggesting a role in inflammatory processes.
Protein Name
VISFATIN
Molecular Weight (kDa)
52.5kD (465 amino acid residues).
Purity
>98% by SDS PAGE and HPLC analysis.
Formulation
10mM HCl
Concentration
Total protein concentration ca.0.1mg/ml after reconstitution.
Preservative
None present
Endotoxin Level
<1EU/ug
Protein Type
Recombinant Protein
Recommended Applications
Functional Assays
Biological activity
The ED 50 was determined by the dose-dependant proliferation of the RPMI 8226 cells. The expected ED 50 for this effect is 15.0-20.0 ng/ml.
Reconstitution
Reconstitute with 0.25ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommends that the vial is gently mixed after reconstitution.

N.B. Due to solubility reasons, it is recommended that the solution be kept at low pH..
Storage/stability
Prior to reconstitution store at +4° C.
After reconstitution store at -20° C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. This protein may be stored for3 months from date of reconstitution.
Menge: 25 ug
Lieferbar: In stock
Listenpreis: 528,00 €
Preis: 528,00 €
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