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Recombinant Human PDCD1/PD1 Protein

Recombinant Human PDCD1/PD1 Protein

ArtNr	230-30062-50
Hersteller	Raybiotech
Menge	50 ug
Quantity options	10 ug 200 ug 50 ug
Kategorie	Oncology By Cell Type Carcinoma Hematology Lymphoma Peptides & Proteins Recombinant Proteins Myelofibrosis Immunoprecipitation Western Blot ELISA Immunostaining Dot Blot
Typ	Proteins
Format	Lyophilized
Applikationen	WB, IP, ELISA, Dot
Specific against	Human (Homo sapiens)
Host	HEK293 cells
Konjugat/Tag	HIS
Purity	>95%
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Versandbedingung	Raumtemperatur
Lieferbar
Manufacturer - Applications	Protein-protein Interaction, Post-translational Modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc.
Manufacturer - Category	Proteins\|Recombinant Proteins\|Human Proteins
Manufacturer - Conjugate / Tag	N-terminal histidine tag
Shipping Temperature	Ambient temperature
Storage Conditions	-20°C
Molecular Weight	Recombinant protein has a calculated molecular weight of about 16 kDa. Due to the abundant glycosylation, it migrates as approximately 30-40 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Description	Recombinant Human PDCD1, derived from the transfected human HEK293 cells.
Purity determined by	SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Gene Symbols	PD1, PDCD1
UNSPSC Code	12352202
Formulation	Lyophilized from a 0.2 µm filtered solution in PBS
Expressed Region	Pro21-Val170
Protein Name & Synonyms	Programmed cell death protein 1 (Protein PD-1) (hPD-1) (CD antigen CD279), Programmed cell death protein 1, Protein PD-1, hPD-1, Programmed Cell Death 1, Systemic Lupus Erythematosus Susceptibility 2, HPD-1, PD1, Programmed Cell Death 1 Protein, CD279 Antigen, CD279, HPD-L, HSLE1, SLEB2, PD-1
Format	Lyophilized powder
Purification	His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration	Determined by Pierce BCA protein assay kit
Reconstitution	Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1 mg/mL with sterile deionized water.
Endotoxin Level	Not determined
Preservative	None
SDS-PAGE Image	Figure 1. Deglycosylation of purified recombinant proteins. Purified proteins were untreated (Lane 2) or treated with protein deglycosylation enzymes under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one major band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated.Lane 1: Protein standard ladder (kDa).Lane 2: Untreated protein under reducing conditions.Lane 3: Treated protein with deglycosylation enzymes under native conditions.Lane 4: Treated protein with deglycosylation enzymes under reducing conditions.Lane 5: Deglycosylation mixture only without target proteins.
Custom Labeling Request	We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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230-30062-50-datasheet.pdf

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Menge: 50 ug

lieferbar

Lieferung vsl. bis 16.10.2025