Recombinant Mouse interleukia IL-16 Protein

Proteins|Recombinant Proteins|Animal Proteins

Ambient temperature

Recombinant protein has a calculated molecular weight of about 14 kDa. Due to the post-translation modifications, it migrates as approximately 14-20 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under native and denature conditions, the protein sizes slightly decreased. See deglycosylation analysis image below.

SDS-PAGE under reducing conditions and visualized by Coomassie blue staining

12352202

Ser1205-Ser1322

Lyophilized powder

Determined by Pierce BCA protein assay kit

Not determined

Figure 1. Deglycosylation analysis of purified recombinant proteins. The same amount of purified proteins were untreated (Lane 2) or treated with protein deglycosylation enzymes under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein size and indicated the untreated recombinant protein (Lane 2) was glycosylated.Lane 1, protein standard ladder (kDa).Lane 2, untreated protein.Lane 3, treated protein with deglycosylation enzymes under native conditions.Lane 4, treated protein with deglycosylation enzymes under denature conditions.Lane 5, deglycosylation enzymes only without target proteins.