Recombinant Human Versican Protein, HEK293 Lysate

Proteins|Recombinant Proteins|Human Proteins

Ambient temperature

Recombinant Human Versican has a calculated molecular mass of 39 kDa. The actual molecular weight may increase slightly due to the potential post-modifications (PTMs).

VCAN

Lyophilized from a 0.2 µm filtered solution in PBS containing mammalian cell protease inhibitor cocktail

Versican, Chondroitin Sulfate Proteoglycan 2, Chondroitin Sulfate Proteoglycan Core Protein 2, Glial Hyaluronate-Binding Protein, Large Fibroblast Proteoglycan, Versican Proteoglycan, CSPG2, GHAP, PG-M, ERVR, WGN1, WGN

Unpurified cell lysate. HEK293 cells transfected with expression vectors harboring target gene were harvested and washed with PBS twice. The cell pastes were re-suspended with ice-cold PBS containing mammalian cell protease inhibitor cocktail and further lysed with freeze-thaw cycles. After clarifying with 20, 000 g centrifugation at 4°C for 30 min, the lysate was aliquoted, lyophilized, and stored at -80°C immediately. Protein concentration was determined by BCA kit (Thermo Scientific, Inc.) using BSA as protein standard. The gene overexpression in lysate was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the lysate derived from HEK293 cells transfected with the empty expression vector was used as a negative control.

Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1 mg/mL with sterile deionized water.