Recombinant Human Amphiphysin Protein, HEK293 Supernatant

Proteins|Recombinant Proteins|Human Proteins

Blue ice

Recombinant Human Amphiphysin has a calculated molecular mass of 76 kDa. The actual molecular weight is about 150 kDa due to the potential post-modifications (PTMs).

AMPH

Culture supernatant of transfected HEK293 cells

Amphiphysin, Amphiphysin, Amphiphysin (Stiff-Mann Syndrome With Breast Cancer 128kD Autoantigen), AMPH1, Amphiphysin (Stiff-Man Syndrome With Breast Cancer 128kDa Autoantigen)phiphysin I

Unpurified cell culture supernatant. HEK293 cells grown in serum-free medium were transfected with expression vector harboring target gene. The cell culture was harvested with centrifugation to remove cells. The cell culture supernatant containing mammalian cell protease inhibitor cocktail was aliquoted and stored at -80 °C immediately. The gene overexpression in culture supernatant was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the culture supernatant derived from HEK293 cells transfected with the empty expression vector was used as a negative control.