Recombinant Human Matrix Metalloproteinase-9 (MMP-9)

Proteins|Recombinant Proteins|Human Proteins|Enzymes & Inhibitors

Ambient temperature

Recombinant protein product has a calculated molecular mass of ˆ¼75 kDa. Due to the abundant glycosylation, it migrates as approximately ˆ¼120 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under native and denature conditions, the protein presented as one reduced ˆ¼75 kDa band. See deglycosylation analysis image below.

12352202

Ala20-Asp707

Lyophilized powder

Determined by Pierce BCA protein assay kit (lot specific)

Not determined

Figure 1. Deglycosylation analysis of purified recombinant proteins. The same amount of purified proteins were untreated (Lane 2) or treated with protein deglycosylation enzymes under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one reduced band at the expected size (ˆ¼75 kDa), thus indicating that the untreated recombinant protein (Lane 2, ˆ¼120 kDa) was glycosylated.Lane 1, protein standard ladder (kDa).Lane 2, untreated protein.Lane 3, treated protein with deglycosylation enzymes under native conditions.Lane 4, treated protein with deglycosylation enzymes under denature conditions.Lane 5, deglycosylation enzymes only without target proteins.