Recombinant Human Interleukin-13 (IL-13) Protein

Proteins|Recombinant Proteins|Human Proteins

Ambient temperature

Recombinant protein product has a calculated molecular mass of 13 kDa. Due to the abundant glycosylation, it migrates as approximately 13-35 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.

SDS-PAGE under reducing conditions and visualized by Coomassie blue staining

12352202

Gly35-Asn146

Lyophilized powder

Determined by BCA protein assay

Figure 1. Deglycosylation of purified recombinant proteins. Purified proteins were untreated (Lane 2) or treated with Protein Deglycosylation Kit under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one major band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated. Lane 1: Protein standard ladder (kDa) Lane 2: Untreated protein under reducing conditions Lane 3: Treated protein with deglycosylation enzymes under native conditions Lane 4: Treated protein with deglycosylation enzymes under reducing conditions.Lane 5: Deglycosylation mixture only without target proteins.