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Recombinant Sus scrofa (pig) Interleukin-4 (IL-4) Protein

Recombinant Sus scrofa (pig) Interleukin-4 (IL-4) Protein

ArtNr	230-30131-50
Hersteller	Raybiotech
Menge	50 ug
Quantity options	10 ug 100 ug 50 ug
Kategorie	Peptides & Proteins Recombinant Proteins Cell Biology Cell Culture Cytokines
Typ	Proteins Recombinant
Format	Lyophilized
Specific against	Wild Boar
Host	HEK293 cells
Konjugat/Tag	HIS
Purity	>95%
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Versandbedingung	Raumtemperatur
Lieferbar
Manufacturer - Category	Proteins\|Recombinant Proteins\|Animal Proteins
Manufacturer - Conjugate / Tag	N-terminal his-tag
Shipping Temperature	Ambient temperature
Storage Conditions	-20°C
Molecular Weight	Recombinant protein product has a calculated molecular mass of 12 kDa. Due to the abundant glycosylation, it migrates as approximately 12-25 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Description	Recombinant Sus scrofa (pig) interleukin-4 (IL-4) protein, derived from the transfected human HEK293 cells.
Purity determined by	SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Gene Symbols	IL4
UNSPSC Code	12352202
Formulation	Lyophilized from a 0.2 µm filtered solution in PBS (pH 7.4)
Expressed Region	His25-Cys133
Protein Name & Synonyms	Interleukin-4, Interleukin 4, IL-4, Lymphocyte Stimulatory Factor 1, B_cell Stimulatory Factor 1, B Cell Growth Factor 1, Binetrakin, Pitrakinra, BSF-1, B-Cell Stimulatory Factor 1, Interleukin 4 Variant 2, BCGF-1, BCGF1, BSF1.
Format	Lyophilized powder
Purification	His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration	Determined by BCA protein assay
Reconstitution	Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water.
SDS-PAGE Image	Figure 1. Deglycosylation of purified recombinant proteins. Purified proteins were untreated (Lane 2) or treated with Protein Deglycosylation Kit under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one major band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated. Lane 1: Protein standard ladder (kDa) Lane 2: Untreated protein under reducing conditions Lane 3: Treated protein with deglycosylation enzymes under native conditions Lane 4: Treated protein with deglycosylation enzymes under reducing conditions.Lane 5: Deglycosylation mixture only without target proteins.
Custom Labeling Request	We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.