4-Epi Doxycycline

Blue Ice

444, 43

Light yellow to yellow solid

An alternative to doxycycline to control gene expression.

Synonyms:
(4R, 4aR, 5S, 5aR, 6R, 12aS)-4-(Dimethylamino)-1, 4, 4a, 5, 5a, 6, 11, 12a-octahydro-3, 5, 10, 12, 12a-pentahydroxy-6-methyl-1, 11-dioxo-2-naphthacenecarboxamide; 6-Deoxy-4-epioxytetracycline

CAS Number:
6543-77-7

Molecular Formula:
C22H24N2O8

Molecular Weight:
444.43

Purity:
≥70%

Form:
Light yellow to yellow solid

Melting Point:
>115°C (dec.)

Method for Determining Identity:
Proton NMR and MS

TLC Conditions:
C18; Isopropanol : Water : Acetic Acid (5 : 4 : 1). Visualized with UV and KMnO4 (single spot; Rf =0.35)

Solubility:
Methanol (Slightly, Heated), Water (Slightly)

Stability:
Unstable in Solution

Storage and Stability:
Store at -20°C under inert atmosphere. For maximum recovery of product, centrifuge the original vial prior to removing the cap.