Ac-Arg-Leu-Arg-AMC (Ac-RLR-AMC, Proteasome Substrate)

Molecular Biology / MB-Fluorescent Labels

-20°C

Highly Purified

38220090

Fluorogenic tri-peptide substrate for measuring the trypsin-like peptidase activity of the 20S proteasome. It is cleaved exclusively at the amide R-AMC bond to release fluorescent product. Shows low Km and high specific activity. This substrate is useful for inhibitor screening and kinetic analysis.

CAS No.:
929903-87-7 (free base)

Excitation:
Excitation: 380nm. Emission: 460nm.

Molecular Formula:
C30H46N10O6

Molecular Weight:
642.8

Sequence:
Acetyl-Arg-Leu-Arg-7-amido-4-methylcoumarin

Solubility:
Soluble in DMSO, methanol or water (aqueous solutions).

Use:
Add from DMSO stock directly to in vitro or in vivo assay at desired concentration. Typical concentrations range from 10-100uM. Activity with purified proteasome: Km=78uM.

Storage and Stability:
Stable for at least 1 year after receipt when stored at -20°C.