Boc-Leu-Arg-Arg-AMC (Boc-LRR-AMC, Proteasome Substrate)

Molecular Biology / MB-Fluorescent Labels

-20°C

Highly Purified

38220090

Fluorogenic tri-peptide substrate for measuring the trypsin-like peptidase activity of the 20S proteasome. Substrate for the Kex2 endopeptidase from S. Cervisiae, which has substrate specificity toward the C-terminal side of LR, PR and RR sequences. Can be cleaved by Kallikrein 5 with a P1 site preference for basic residues (R and K). This substrate is useful for inhibitor screening and kinetic analysis.

CAS Number:
109358-46-5 (free base)

Source:
Synthetic

Excitation:
Excitation: 380nm. Emission: 460nm.

Use:
Add from DMSO stock directly to in vitro or in vivo assay at desired concentration. Typical concentrations range from 10-100uM.

Molecular Formula:
C33H52N10O7 . HCl

Molecular Weight:
773.8 . 36.5

Sequence:
Boc-Leu-Arg-Arg-7-amido-4-methylcoumarin

Solubility:
5% acetic acid

Purity:
≥98%

Appearance:
Supplied as a solid lyophilized powder.

Storage and Stability:
Stable for at least 1 year after receipt when stored at -20°C.