Agarose High Gel Temp

Molecular Biology / MB-BioSep Agarose

RT

White, homogeeous free flowing powder

High Gel Strength Agarose Combines very high gel strength with low EEO and is a good choice for high speed, high temperature pulsed field gel electrophoresis applications.

Applications:
• Pulsed Field Electrophoresis
• Immunoelectrophoresis
• Blotting Assays (Southern, northern)
• Analytical DNA Gels ≥ 1000bp
• Preparative Electrophoresis
• For use with all buffer systems

EEO:
≤0.14

Melting Point (1.5%):
85.5-88.5°C

Gel Point (1.5%):
39.5-42.5°C

Gel Strength (1.5%):
≥1200g/cm2

Clarity:
≤50 NTU

Sulfate:
≤0.2%

RNase:
None Detected

DNase:
None Detected

Quality Control:
Genetic tested for a wide array of molecular biology procedures ensuring high recovery of biologically active DNA, high cloning efficiency and consistent reproducibility.

Functional and Molecular Biology Testing: To Pass Test
• Comparative assay of different size DNA fragments
• Southern Blot (125-23, 000bp)
• Background Fluorescence (TAE/TE, EtBr):
• DNA Binding (TAE, EtBr)
• Restriction Ligation Assay (Bam HI, Hinc II, EcoR I, Hind III, Pst I, and T4 DNA Ligase)

Recommended Usage (1X TAE):
ConcentrationMolecular Weight
0.4-2.0%50-500kb

Storage and Stability:
Store powdered agarose at RT. Opened bottles should be capped tightly and kept in a low humidity environment. For greater separation capacity, keep the gel at 4°C for 1 hour before use.