Reproductive Engineering Techniques in Mice Technical Manual

Written by Dr. Naomi Nakagata, this beautifully bound, full color manual offers step-by-step protocols with illustrative pictures, media composition, and references. It is the perfect companion piece to accompany your mouse IVF work performed with KYD and CSR products.
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Reproductive Engeneering Techniques in Mice (3rd Edition)

TABLE OF CONTENTS
Chapter 1 In Vitro Fertilization (IVF)

• 1-1 Preparing and Assembling Pipettes for Embryo Handling p.4
• 1-2 In Vitro Fertilization (IVF) 6 1-3 In Vitro Fertilization (IVF) using Ultra-Superovulation Reagent p.12

Chapter 2 Transportation of sperm

• 2-1 Collection and Transport at Cold Temperature of Cauda Epididymis p.14
• 2-2 In Vitro Fertilization using Epididymal Sperm Transported at Cold Temperature p.18

Chapter 3 Cryopreservation of sperm

• 3-1 Cryopreservation of Mouse Spermatozoa p.20
• 3-2 In Vitro Fertilization using Cryopreserved Spermatozoa p.26
• 3-3 Rescue In Vitro Fertilization Method for Legacy Stock of Cryopreserved Spermatozoa p.32

Chapter 4 Preparation of Oocyte & Embryo

• 4-1 Preparation of Laser-microdissected Oocytes p.36
• 4-2 Partial Zona Dissection (PZD) p.39
• 4-3 Collecting 2-Cell Stage Embryos p.42

Chapter 5 Transportation of Oocyte & Embryo

• 5-1 Transport of 2-Cell Embryos at Cold Temperature p.46
• 5-2 Transportation of Mouse Oviducts Containing 2-Cell Embryos at Low Temperature p.52

Chapter 6 Cryopreservation of Oocyte & Embryo

• 6-1 Simple Vitrification of Mouse Embryos p.54
• 6-2 Simple Vitrification of Mouse Oocytes p.59
• 6-3 Vitrification and Transplantation of Mouse Ovaries p.62

Chapter 7 Other Techniques

• 7-1 Vasectomy for the Creation of Sterile Males p.64
• 7-2 Embryo Transfer into the Oviduct p.66
• 7-3 Embryo Transfer into the Uterus p.72
• 7-4 Caesarean Section and Fostering p.76

Chapter 8 Media

• 8-1 Storage of Media and Solutions in Ampoules Under Nitrogen Gas p.78
• 8-2 Tables of Media Composition p.79

All material:Copyright © 2015 by Naomi Nakagata