BCIP/NBT One Component Membrane Alkaline Phosphatase (AP) Substrate

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This BCIP/NBT 1 component buffered alkaline phosphatase substrate (5-bromo-4-chloro-3-indolyl-phosphate and nitroblue tetrazolium) is a precipitating substrate used with the enzyme alkaline phosphatase designed for various membrane immunoassays but not recommended for microwell or immunohistochemical applications. Initially, the unreacted substrate should be colorless to light yellow in appearance. When this substrate system is reacted with alkaline phosphatase, an insoluble, permanent dark purple reaction product is obtained.
Leinco Technologies’ BCIP/NBT 1 Component Membrane Substrate is a highly sensitive, high performance formulation that produces a high signal to noise ratio with low membrane background. The outstanding shelf life of at least thirty six months for the BCIP/NBT 1 Component Membrane Substrate makes this reagent ideal for long term use of the same manufacturing lot.