Rat Ephrin-B1 / EFNB1 Human Cells Transfected Lysate (positive control) (denatured)

Hersteller Sino Biological
Kategorie
Typ Cell Lysate
Specific against Human, Rat
Format Human Cells
Menge 300 ug
Host Human
ArtNr 80106-R08HL-300
eClass 6.1 32190290
eClass 9.0 32190290
Lieferbar
Molecule
EFNB1
Product Description
Human Cells transfected lysate in which Rat EFNB1 / Ephrin-B1 has been over-expressed. The whole cell lysate is provided in 1X Sample Buffer.
Expressed Host
Human Cells
Preparation Method
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis Buffer
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200uL pure water and boiled for 2-5 min. 3. Store it at -80C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles. Notes: The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer
Storage Instruction
Store at -80C. Aliquot to avoid repeated freezing and thawing
Stability
Samples are stable for up to twelve months from date of receipt at -80C
Application notes
WB: Use at an assay dependent dilution. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Menge: 300 ug
Lieferbar: In stock
Listenpreis: 253,59 €
Preis: 253,59 €
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