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Recombinant Human c-MET/HGFR Protein (aa 956-1390, His & GST Tag)

Recombinant Human c-MET/HGFR Protein (aa 956-1390, His & GST Tag)

ArtNr	10692-H20B1-4
Hersteller	Sino Biological
Menge	4 mg
Quantity options	1 mg 2 mg 4 mg 50 ug
Kategorie	Oncology Neuroscience Neural Lineage Markers Molecular Targets for Neuroscience Targets for Rodent Neurosciences By Cell Type Blastoma Carcinoma Glioma Hematology Lymphoma Myeloma Melanoma Sarcoma Peptides & Proteins Recombinant Proteins Adenocarcinoma Mesothelioma Astrocyte Markers
Typ	Proteins
Specific against	Human (Homo sapiens)
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	AUTS9, c-Met, DFNB97, HGFR, RCCP2
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Manufacturer - Category	Protein
Shipping method	Blue ice shipping
Molecule	MET
Synonym	AUTS9, c-Met, DFNB97, HGFR, RCCP2
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Protein Construction	A DNA sequence encoding the human MET (P08581-1) (Lys956-Ser1390) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Expressed Host	Baculovirus-Insect Cells
Purity	> 97 % as determined by SDS-PAGE
Bio-Activity	1. The specific activity was determined to be 10 nmol/min/mg using MBP as substrate. 2. Measured by its binding ability in a functional ELISA. Immobilized human HGFR (aa 956-1390) (Cat: 10692-H20B1) at 10 ug/ml (100 ul/well) can bind biotinylated human HGF-his (Cat: 10463-H08H) with a linear range of 15.6-125 ng/ml.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Met
Molecule Mass	The recombinant human MET /GST chimera consists of 672 amino acids and has a calculated molecular mass of 76.8 kDa. The recombinant protein migrates as an approximately 68 kDa band in SDS-PAGE under reducing conditions.
Formulation	Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol, 3mM DTT
Accession	P08581-1
Amino acid sequence	Lys956-Ser1390
TAG	N-GST & His

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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10692-H20B1-4-datasheet.pdf

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Menge: 4 mg

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