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Recombinant Human CD70/CD27 Ligand Protein (hFc Tag)

Recombinant Human CD70/CD27 Ligand Protein (hFc Tag)

ArtNr	10780-H01H-1
Hersteller	Sino Biological
Menge	1 mg
Quantity options	1 mg 100 ug 2 mg 4 mg
Kategorie	Oncology By Cell Type Carcinoma Hematology Lymphoma Peptides & Proteins Recombinant Proteins
Typ	Proteins
Specific against	Human (Homo sapiens)
Host	Human
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	CD27L, CD27LG, TNFSF7
Similar products	CD70
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Manufacturer - Category	Protein
Molecule	CD70
Synonym	CD27L, CD27LG, TNFSF7
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction	A DNA sequence encoding the extracellular domain of human CD70 (NP_001243.1) (Gln 39-Pro 193) was fused with the Fc region of human IgG1 at the N-terminus via a polypeptide linker.
Expressed Host	Human Cells
Purity	(72.6+18.6) % as determined by SDS-PAGE
Bio-Activity	Measured by its binding ability in a functional ELISA. 1. Immobilized mouse CD27 at 2 ug/mL (100 ul/well) can bind human CD70 with a linear range of 78-1250 pg/ml. 2. Immobilized mouse CD27 / Fc at 2 ug/mL (100 ul/well) can bind biotinylated human CD70 with a linear range of 0.39-12.5 ng/ml. 3. Immobilized human CD27 / Fc at 2 ug/mL (100 ul/well) can bind biotinylated human CD70 with a linear range of 0.39-12.5 ng/ml.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Glu 20
Molecule Mass	The recombinant human CD70/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 413 amino acids and predicts a molecular mass of 45.5 kDa. As a result of different glycosylation, the rh CD70/Fc migrates as several bands with the approximate molecular mass of 55-60, 110-120 and 160-170 kDa corresponding to the monomeric, dimeric and trimeric form respectively in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4
Accession	NP_001243.1
Amino acid sequence	Gln39-Pro193
TAG	N-human IgG1-Fc

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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10780-H01H-1-datasheet.pdf

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