Tubulin, beta 3 (Tubulin beta III, TUBB 3, TUBB3, Neuron Specific beta III Tubulin, beta-4, MC1R, Tubulin beta 4, TUBB 4)

Antibodies / Antibodies-Tubulin

-20°C

Supplied as a liquid in PBS, 1% BSA, 15mM sodium azide.

Synthetic peptide corresponding to aa443-450, ESESQGPK, of human class lll beta-tubulin. KLH conjugated. Epitope: C-terminus.

Class 3 beta tubulin (beta 3-tubulin) is a vertebrate tubulin Isotype specific to the neurons and mammalian testis cells, making it an ideal neuronal marker. Overexpression of class 3 beta tubulin is associated with the resistances of microtubule- targeted cancer drugs in lung cancer cell lines, breast cancer cell lines, and ovarian tumors.

Applications:
Suitable for use in Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot and ELISA. Other applications have not been tested.

Recommended Dilutions:
Immunocytochemistry (Fixed): 1:100-1:500.
Immunohistochemistry (FFPE): 1:20-1:100. Requires HIER using sodium citrate buffer pH6.0 prior to staining.
Western Blot: 1:1000.
Optimal dilutions to be determined by the researcher.

Positive Control:
Brain tissue or neurons of any type.

Negative Control:
Non-neuronal tissue or Astrocytes.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.