Tubulin, beta IV (Tubulin beta 4, TUBB 4, TUBB4, Beta-4, Beta-4 Tubulin, MC1R, Tubulin beta 3)

Antibodies / Antibodies-Tubulin

-20°C

Supplied as a liquid, 15mM sodium azide.

Synthetic peptide corresponding to the C-terminal sequence of human Tubulin, beta IV (BSA). Cellular Localization: Centrosomal.

The major building block of microtubules is tubulin, an intracellular cylindrical filamentous structure that is present in almost all eukaryotic cells. Except in the simplest eukaryotes, tubulin exists in all cells as a 100kD protein, a heterodimer of two similar but not identical polypeptides, designated alpha and beta, that assembles into microtubules. Both alpha and beta tubulins consist of various isotypes. For beta tubulin, six evolutionarily conserved isotypes were identified (designated beta I-beta VI). Beta IV exists as two subtypes in mammals, differing from each other at 10 positions. beta IVa is brain specific, whereas beta IVb is ubiquitous, and both appear to be constitutive. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis, organellar and flagellar movement, intracellular transport, and cytoskeletal functions.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: paraffin sections
Western Blot: 1:400 detects a band at ~55kD and possibly the 100kD dimer.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.