ERAP2 (Endoplasmic Reticulum Aminopeptidase 2, Leukocyte-derived Arginine Aminopeptidase, L-RAP, LRAP)

Antibodies / Antibodies-Amyloid

-20°C

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Synthetic peptide corresponding to C-QYLKKFSYRNAKND, from human ERAP2, at the internal region of the protein (NP_071745.1).

Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Preferentially hydrolyzes the basic residues Arg and Lys.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:16, 000
Western Blot: 0.5-1.5ug/ml, observed in human spleen lysates on ~110kD bands
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.