CD146 (MUC18, Muc-18, MCAM, Mel-CAM, S-endo, Melanoma Cell Adhesion Molecule) (PE)

Antibodies / Antibodies-CD Markers

4°C Do not freeze

Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide. Labeled with R-Phycoerythrin (PE).

HUVEC

CD146, also known as MCAM (melanoma cell adhesion molecule) or MUC18, is a heavily glycosylated transmembrane glycoprotein with more than 50% of the mass from carbohydrates. It is expressed on epithelial and endothelial cells, fibroblasts, multipotent mesenchymal stromal cells, activated T cells and activated keratinocytes, and on some cancer cells, especially melanoma. The presence of CD146 on circulating blood cells has been confined to the activated T cells rather than circulating endothelial cells. CD146 mediates heterophilic cell adhesion and regulates monocyte transendothelial migration.

Applications:
Suitable for use in Flow Cytometry. Other applications have not been tested

Recommended Dilution:
Flow Cytometry: 10ul labels 10e6 cells in 100ul or 100ul whole blood
Optimal dilution determined by the researcher.

Excitation Laser:
Blue (488nm)

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.