MALT1 (Paracaspase, Mucosa-associated Lymphoid Tissue Lymphoma Translocation Protein 1)

Antibodies / Antibodies-Apoptosis

-20°C

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138567-AF.

Recombinant protein corresponding to aa701-808 from human MALT1.

Mucosa associated lymphoid tissue lymphoma translocation gene 1 (MALT1) is found in extranodal low-grade B cell lymphomas. MALT1 encodes two Ig-like C2-type domains and fuses with an API2 gene, which is highly expressed in adult lymphoid tissue. The translocation of this MALT1 gene, which maps to human chromosome 18q21, and the apoptosis-inhibiting API2 gene results in an increased development of MALT lymphomas and apoptosis inhibition. Sites at which this API2-MALT1 (11; 18)(q21; q21) translocation commonly occurs are within human lung and kidney tissue. MALT lymphoma expresses nuclear Bcl10, which mediates the oligomerization and activation of a MALT1 caspase-like domain. MALT1 mRNA is found in pre-B cells, mature B cells and plasma cells.

Applications:
Suitable for use in Western Blot, Flow Cytometry, Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 0.5-1ug/10e6 cells
Immunofluorescence: 1-2ug/ml
Western Blot: 0.5-1ug/ml
Immunohistochemistry (Formalin-fixed): 0.5-1ug/ml for 30min at RT
Requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 minutes
Optimal dilutions to be determined by the researcher.

Positive Control:
Jurkat, Daudi or HeLa cells. Tonsil or lymphoma.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.