Bcl 10 (B Cell Leukemia 10, CIPER, mE10, CARMEN, CLAP)

Antibodies / Antibodies-Apoptosis

-20°C

Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

C-terminal sequence [EMFLPLRSRTVSRQC] of Bcl10.

Apoptosis is related to many diseases including cancer. Cell death signals are transduced by death domain (DD) and caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include ARC, RAIDD, Apaf-1, caspase 9, and caspase 2. A novel CARD containing protein was recently identified by several groups and designated Bcl10, CIPER, mE10, CARMEN, CLAP (1-6). Bcl 10 is a cellular homolog of the equine Herpes virus-2 E-10 gene. Overexpression of Bcl10 induces JNK, p38, and NF-kB activation. Bcl 10 interacts with caspase 9 and enhances pro-caspase-9 processing and induces apoptosis through caspase 9 activation. Bcl 10 exhibits a variety of mutations in MALT lymphomas and in B and T cell lineage lymphomas indicating that it may be commonly involved in the pathogenesis of human malignancy . Bcl 10 is expressed in many human and murine tissues and cell lines.

Bcl 10 is identified through its direct involvement in t(1; 14)(p22; q32) of mucosa-associated lymphoid tissue (MALT) lymphoma. It is also expressed both in normal lymphoid tissue but doesn’t exhibit crossreactivity with other tissues except breast. In non-malignant B-cell follicles the protein is expressed in the cytoplasm of the germinal center B-cells, while in MALT lymphomas with t(1; 14)(p22; q32), Bcl 10 expression can be detected both in nucleus and cytoplasm.

Applications:
Suitable for use in Western Blot. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1:250-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.