NIFK, Mouse, aa75-90 (Ki-67ip)

Antibodies / Antibodies-Nuclear Proteins

-20°C

Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.

Synthetic peptide corresponding to amino acids 75-90 of mouse NIFK.

NIFK is a nucleolar protein that interacts with Ki67, a protein strongly associated with, and a specific marker of, cell proliferation. The marsupial counterpart of human NIFK was recently identified and suggests that NIFK may be involved in the organization of higher order chromatin structure. NIFK was found to interact with the forkhead associated domain of Ki67 in mitotic cells and the interaction is thought to be phosphorylation-dependent.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
observed size: 34 kD
positive control: NIH3T3 whole cell lysate
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.