SARS, Nucleocapsid Protein (Severe Acute Respiratory Syndrome)

Antibodies / Antibodies-Infectious Disease SARS

-20°C

Supplied as a liquid in PBS, 0.05% sodium azide.

Synthetic peptides corresponding to amino acids 399-411 of putative SARS nucleocapsid (Genbank accession no. NP_828858).

It has recently been shown that SARS is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kD protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46kD nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics.

Applications:
Suitable for the detection of SARS Nucleocapsid protein in western blot analysis. It can also be used in cell staining. Western blot analysis of SARS Nucleocapsid in (A) untransfected mouse melanoma cell lysate and (B) transfected cell lysate using antibody at a 1:2000 dilution.

Recommended Dilution:
Western blot: 1:100-1:2000

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.