IgG, H&L, X-Adsorbed (FITC)

Pab

F(ab’)2

-20°C

Supplied as a liquid in 10mM sodium phosphate, 0.15M sodium chloride, pH7.2, 1% BSA (Protease/IgG free), 0.05% sodium azide. labeled with Fuorescein Isothiocyanate (FITC).

Purified Rabbit IgG, whole molecule

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry, Immunocytochemistry and Immunomicrosopy. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:20-1:2000
Immunocytochemistry: 1:50-1:5000
Optimal dilutions to be determined by the researcher.

Absorption:
494nm

Emission:
518nm

FITC/Protein Ratio:
3-7 Moles FITC per Mole Antibody

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.