Cross-linked C-Telopeptide of Type I Collagen (CTXI) BioAssay™ ELISA Kit (Human)

Blue Ice

38220000

The Cross-linked C-Telopeptide of Type I Collagen (CTXI) BioAssay™ ELISA Kit (Human) is a competitive inhibition immunoassay for the in vitro quantitative measurement of CTXI in human plasma, serum and other biological fluids.

Detection Range:
123.5-10, 000pg/ml

Sensitivity:
52.9pg/ml

Precision:
Intra-Assay CV: <10%
Inter-Assay CV: <12%

Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific for CTXI has been pre-coated onto a microplate. A competitive inhibition reaction occurs between biotin-labeled CTXI and unlabeled CTXI (standards or samples) for limited binding sites on the pre-coated antibody. After incubation, the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is inversely proportional to the concentration of CTXI in the sample. After addition of the substrate solution, the intensity of color developed is inversely proportional to the concentration of CTXI in the sample.

Kit Components:
*024465A: Microtiter Plate, 96 wells, Pre-coated, ready to use.
*024465B: Standard, 2x1vial
024465C: Standard Diluent, 1x20ml
*024465D: Detection Reagent A, 1x1vial
*024465E: Detection Reagent B, 1x120ul
024465F: Assay Diluent A, 1x12ml
024465G: Assay Diluent B, 1x12ml
024465H: TMB Substrate, 1x9ml
024465K: Stop Solution, 1x6ml
024465L: Wash Buffer, 30X, 1x20ml
024465M: Reagent Diluent, 1x300ml

Storage and Stability:
Store *024465A, *024465B, *024465D and *024465E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Summary:
1. Prepare all reagents, samples and standards.
2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C.
3. Aspirate and wash 3 times.
4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.
5. Aspirate and wash 5 times.
6. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37°C.
7. Add 50ul Stop Solution. Read absorbance at 450nm immediately.