Pentosidine (PTD) BioAssay™ ELISA Kit

Kits and Assays / Kits-ELISA, BioAssay™

4°C/-20°C

The Pentosidine (PTD) ELISA Kit is a competitive inhibition immunoassay for in vitro quantitative measurement of PTD in serum, plasma and other biological fluids.

Detection Range:
4.94-400ng/ml

Sensitivity:
2.28ng/ml

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components:
*027322A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use
*027322B: Standard, 2x1vial
027322C: Standard Diluent, 1x20ml
*027322D: Detection Reagent A, 1x120ul
*027322E: Detection Reagent B, 1x120ul
027322F: Assay Diluent A, 2X, 1x12ml
027322G: Assay Diluent B, 2X, 1x12ml
027322H: TMB Substrate, 1x9ml
027322K: Stop Solution, 1x6ml
027322L: Wash Buffer, 30X, 1x20ml

Storage and Stability:
Store *027322A, *027322B, *027322D and *027322E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary:
1. Prepare all reagents, samples and standards.
2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C.
3. Aspirate and wash 3 times.
4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.
5. Aspirate and wash 5 times.
6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C.
7. Add 50ul Stop Solution. Read at 450nm immediately.