Secretin (SCT) BioAssay™ ELISA Kit (Mouse)

Kits and Assays / Kits-ELISA, BioAssay™

4°C/-20°C

Secretin (SCT) BioAssay™ ELISA Kit (Mouse) is a competitive inhibition immunoassay for in vitro quantitative measurement of SCT in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Detection Range:
12.35-1000pg/ml

Sensitivity:
4.95pg/ml

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components:
*027984A: Microtiter Plate, 96 wells, Pre-coated, ready to use
*027984B: Standard, 2x1vial
027984C: Standard Diluent, 1x20ml
*027984D: Detection Reagent A (green), 1x120ul
*027984E: Detection Reagent B (red), 1x120ul
027984F: Assay Diluent A, 1x12ml
027984G: Assay Diluent B, 1x12ml
027984H: TMB Substrate, 1x9ml
027984K: Stop Solution, 1x6ml
027984L: Wash Buffer, 30X, 1x20ml

Precaution:
The Stop Solution (027984K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability:
Store *027984A, *027984B, *027984D and *027984E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary:
1. Prepare all reagents, samples and standards;
2. Add 50ul standard or sample to each well.
3. Add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.
6. Aspirate and wash 5 times.
7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C.
8. Add 50ul Stop Solution. Read at 450nm immediately.