Tryptase (TPS) BioAssay™ ELISA Kit (Human)

Kits and Assays / Kits-ELISA, BioAssay™

4°C/-20°C

The Tryptase (TPS) BioAssay™ ELISA Kit is a quantitative sandwich assay for the detection of TPS in human serum, plasma and other biological fluids. Cell lysates and tissue homogenates, though not tested, may potentially be used as samples.

Detection Range:
0.156-10ng/ml

Sensitivity:
<0.094ng/ml

Kit Components:
*359440A: Microtiter Strips, 1x48 wells or 1x96 wells (8x12 wells).
*359440B: Standard, 2x1 vial
359440C: Sample/Standard Dilution Buffer, 1x10ml or 1x20ml
359440D: Antibody (Biotin) (Concentrated), 1x60ul or 1x120ul
359440E: Antibody Dilution Buffer, 1x5ml or 1x10ml
359440F: Streptavidin (HRP) (SABC), 1x60ul or 1x120ul
359440G: SABC Dilution Buffer, 1x5ml or 1x10ml
359440H: TMB Substrate, 1x5ml or 1x10ml
359440J: Stop Solution, 1x5ml or 1x10ml
359440K: Wash Buffer, 25X, 1x15ml or 1x30ml

Storage and Stability:
Store unopened *359440A at 4ºC; store at -20ºC once opened. Store unopened *359440B at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Summary:
1. Wash plate 2 times before adding standards and samples to wells!
2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37°C. Aspirate and wash 2 times.
3. Add 100ul Antibody (Biotin) working solution to each well and incubate for 60 minutes at 37°C.
4. Aspirate and wash 3 times.
5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C
6. Aspirate and wash 5 times.
7. Add 90ul TMB substrate. Incubate 15-30 minutes at 37°C
8. Add 50ul Stop Solution. Read at 450nm immediately.
9. Calculate results.