SPFH2, Recombinant, Human, aa25-339, His-Tag (SPFH Domain-containing Protein 2, ERLIN2, C8orf2, Erlin-2, NET32, SPG18)

Blue Ice

37, 8

Supplied as a liquid in 20mM tris-HCl buffer, pH 8.0, 2M urea, 20% glycerol.

ERLIN2, also known as SPFH2, is SPFH domain-containing proteins that belong to the prohibitin family. Also, this protein is a ubiquitously expressed 339 amino acid protein that belongs to the band 7/mec-2 family. Localized to lipid raft-like domains in the membrane of the endoplasmic reticulum (ER), ERLIN2 plays a crucial role in the ER-associated degradation (ERAD) pathway that removes metabolically regulated and aberrant proteins from the ER.

Source:
Recombinant protein corresponding to aa25-339 from human ERLIN2 at N-terminal, fused to His-tag expressed in E.coli.

Molecular Weight:
~37.8kD (338aa)

Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MGSKIEEGHI GVYYRGGALL TSTSGPGFHL MLPFITSYKS VQTTLQTDEV KNVPCGTSGG VMIYFDRIEV VNFLVPNAVY DIVKNYTADY DKALIFNKIH HELNQFCSVH TLQEVYIELF DQIDENLKLA LQQDLTSMAP GLVIQAVRVT KPNIPEAIRR NYELMESEKT KLLIAAQKQK VVEKEAETER KKALIEAEKV AQVAEITYGQ KVMEKETEKK ISEIEDAAFL AREKAKADAE CYTAMKIAEA NKLKLTPEYL QLMKYKAIAS NSKIYFGKDI PNMFMDSAGS VSKQFEGLAD KLSFGLEDEP LETATKEN

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.