Amylase Alpha 2, Pancreatic (AMY2) Recombinant, Human

Blue Ice

31, 7

Supplied as lyophilized powder from PBS, pH 7.4, 0.01% sarcosyl, 5% trehalose. Reconstitute with sterile 10mM PBS, pH7.4 to a concentration of 0.1-1mg/ml. Do not vortex.

Recombinant protein corresponding to aa188-432 with an N-Terminal His Tag from human AMY2, expressed in E. coli. Uniprot/Accession: P04746.

Amino Acid Sequence:
DYV RSKIAEYMNH LIDIGVAGFR LDASKHMWPG DIKAILDKLH NLNSNWFPAG SKPFIYQEVI DLGGEPIKSS DYFGNGRVTE FKYGAKLGTV IRKWNGEKMS YLKNWGEGWG FVPSDRALVF VDNHDNQRGH GAGGASILTF WDARLYKMAV GFMLAHPYGF TRVMSSYRWP RQFQNGNDVN DWVGPPNNNG VIKEVTINPD TTCGNDWVCE HRWRQIRNMV IFRNVVDGQP FTNWYDNGSN QV

Predicted Isoelectric Point:
9.0

Pedicted Molecular Weight:
31.7kD

Accurate Molecular Mass:
32kD as determined by SDS-PAGE reducing conditions.

Subcellular Location:
Secreted, Extracellular matrix

Applications:
Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile sterile 10mM PBS pH7.4. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Note:
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.