Anterior Gradient Protein 2 (AGR2) Recombinant, Mouse

Blue Ice

23, 6

Supplied as lyophilized powder from 20mM Tris, , 50mM sodium chloride, pH 8.0, 0.01% sarcosyl, 5% trehalose. Reconstitute with 20mM Tris, 150mM sodium chloride, pH 8.0 to a concentration of 0.1-1mg/ml. Do not vortex.

Recombinant protein corresponding to aa21-175 of mouse Anterior Gradient Protein 2 with a N-terminal His-tag expressed in E. coli. Swiss/UniProt Accession: O88312

Purity:
≥95%

Amino Acid Sequence:
MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKAMADIGSEF-KDTTVKSGAK KDPKDSRPKL PQTLSRGWGD QLIWTQTYEE ALYRSKTSNR PLMVIHHLDE CPHSQALKKV FAEHKEIQKL AEQFVLLNLV YETTDKHLSPDGQYVPRIVF VDPSLTVRAD ITGRYSNRLY AYEPSDTALL YDNMKKALKL LKTEL

Molecular Weight:
23.6kD

Applications:
Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C..Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note:
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.