Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1) Recombinant, Human

Blue Ice

31, 1

Supplied as a lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 0.01% sarcosyl, 5% Trehalose. Reconsitute with sterile dH2O to a concentration of 0.1-1mg/ml. Do not vortex.

Recombinant protein corresponding to
aa180-427 with an N-terminal His tag from human DGAT1 expressed in E. coli. Swiss/UniProt Accession: O75907.

Amino Acid Sequence:
A AVVLLVESIT PVGSLLALMA HTILFLKLFS YRDVNSWCRR ARAKAASAGK KASSAAAPHT VSYPDNLTYR DLYYFLFAPT LCYELNFPRS PRIRKRFLLR RILEMLFFTQ LQVGLIQQWM VPTIQNSMKP FKDMDYSRII ERLLKLAVPN HLIWLIFFYW LFHSCLNAVA ELMQFGDREF YRDWWNSESV TYFWQNWNIP VHKWCIRHFY KPMLRRGSSK WMARTGVFLA SAFFHEYLVS VPLRMFR

Predicted Molecular Mass:
30.8kD

Predicted Isoelectric Point:
10.0

Accurate Molecular Mass:
27kD as determined by SDS-PAGE reducing conditions

Subcellular Localization:
Endoplasmic reticulum lumen

Applications:
Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -70°C. Aliquots are stable for at least 12 months from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note:
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.