Ectonucleotide Pyrophosphatase/Phosphodiesterase 2 (ENPP2) Recombinant, Human

Blue Ice

35

Supplied as lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 1mM EDTA, 1mM DTT, 0.01% sarcosyl, , 5% trehalose, 0.05% Proclin-300. Reconstitute with 20mM Tris, 150mM sodium chloride, pH 8.0. to a concentration of 0.1-1mg/ml. Do not vortex.

Recombinant protein corresponding to aa637-905 of human ENPP2, with N-terminal His-tag, expressed in E. coli. Uniprot/Accession: Q13822.

Amino Acid Sequence:
RLHT KGSTEERHLL YGRPAVLYRT RYDILYHTDF ESGYSEIFLM PLWTSYTVSK QAEVSSVPDH LTSCVRPDVR VSPSFSQNCL AYKNDKQMSY GFLFPPYLSS SPEAKYDAFL VTNMVPMYPA FKRVWNYFQR VLVKKYASER NGVNVISGPI FDYDYDGLHD TEDKIKQYVE GSSIPVPTHY YSIITSCLDF TQPADKCDGP LSVSSFILPH RPDNEESCNS SEDESKWVEE LMKMHTARVR DIEHLTSLDF FRKTSRSYPE ILTLK

Predicted Molecular Weight:
35.0kD

Predicted Isoelectric Point:
6.5

Predicted Molecular Weight:
34.8kD

Accurate Molecular Mass:
35kD as determined by SDS-PAGE reducing conditions.

Subcellular Location:
Secreted

Applications:
Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Note:
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.