Glucuronidase Beta (GUSb) Recombinant, Human

Blue Ice

27, 1

Supplied as lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 5% trehalose, 0.01% sarcosyl. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0 to a concentration of 0.1-1mg/ml. Do not vortex.

Recombinant Human from E. coli
Recombinant protein corresponding to
Glu451-Leu649 of human Glucuronidase Beta with a N-terminal His-Tag expressed in E. coli. Swiss/UniProt Accession: P08236

Amino Acid Sequence:
EPASHLESAG YYLKMVIAHT KSLDPSRPVT FVSNSNYAAD KGAPYVDVIC LNSYYSWYHD YGHLELIQLQ LATQFENWYK KYQKPIIQSE YGAETIAGFH QDPPLMFTEE YQKSLLEQYH LGLDQKRRKY VVGELIWNFA DFMTEQSPTR VLGNKKGIFT RQRQPKSAAF LLRERYWKIA NETRYPHSVA KSQCLENSL

Predicted Isoelectric Point:
8.7

Predicted Molecular Weight:
27.1kD

Accurate Molecular Mass:
27kD as determined by SDS-PAGE reducing conditions

Subcellular Location:
Lysosome

Applications:
Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -70°C. Stable for 6 months after receipt. Reconstitute with sterile 20mM Tris, 150mM sodium chloride. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Note:
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.