Myosin Light Chain 1, Alkali, Fast Skeletal (MYL1) Recombinant, Human

Blue Ice

16, 8

Supplied as lyophilized powder from 20mM Tris, 150mM sodium chloride pH 8.0, 0.01% sarcosyl, 5% Trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml. Do not vortex.

Recombinant protein corresponding to Phe3-Ala142 with N-Terminal His Tag from human MYL1, expressed in E. coli Swiss/UniProt Accession: P05976.

Amino Acid Sequence:
FSADQIAE FKEAFLLFDR TGDSKITLSQ VGDVLRALGT NPTNAEVRKV LGNPSNEELN AKKIEFEQFL PMMQAISNNK DQATYEDFVE GLRVFDKEGN GTVMGAELRH VLATLGEKMK EEEVEALMAG QEDSNGCINY EA

Predicted Isoelectric Point:
4.9

Predicted Molecular Weight:
16.8kD

Accurate Molecular Mass:
17kD as determined by SDS-PAGE under reducing conditions

Subcellular Location:
Secreted

Applications:
Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Note:
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.