Neurofilament, Light Polypeptide (NEFL) Recombinant, Mouse

Blue Ice

12, 6

Supplied as lyophilized powder from PBS, pH 7.4, 1mM DTT, 5% trehalose, 0.01% sarcosyl, 0.05% Proclin-300. Reconstitute with sterile PBS to a concentration of 0.1-1mg/ml. Do not vortex.

Source:
Recombinant protein corresponding to aa139-234 (P08551) with a N-Terminal His-Tag from mouse NEFL expressed in E. coli

Predicted Molecular Weight:
12.6kD

Predicted Isoelectric Point:
4.8

Amino Acid Sequence:
MGHHHHHHSGSEF-YEQEIRDLRL AAEDATNEKQ ALQGEREGLE ETLRNLQARY EEEVLSREDA EGRLMEARKG ADEAALARAE LEKRIDSLMD EIAFLKKVHE EEIAEL

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation and SDS-PAGE.
Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Included Protein Molecular Weight Marker:
168631: Protein Molecular Weight Marker, Unstained Ready to use, No heating, diluting or reducing agents needed.
MW: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD, 70kD
Double intensity: 10kD, 18kD, 26kD
Supplied in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Add 3-5ul/well for mini gels; 7ul/well for large gels. Store at -20°C Stable for 6 months after receipt.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note:
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.