Pyruvate Kinase, Muscle (PKM2) Recombinant, Human

Blue Ice

51, 2

Supplied as lyophilized powder from PBS, pH7.4, 0.01% sarcosyl, 5% trehalose. Reconstitute with PBS, pH 7.4 to a concentration of 0.1-1mg/ml. Do not vortex.

Recombinant protein corresponding to aa294-470 from human PKMS with an N-Terminal His and GST Tag, expressed in E. coli. Uniprot/Accession: P14618.

Amino Acid Sequence:
RGDLGIE IPAEKVFLAQ KMMIGRCNRA GKPVICATQM LESMIKKPRP TRAEGSDVAN AVLDGADCIM LSGETAKGDY PLEAVRMQHL IAREAEAAIY HLQLFEELRR LAPITSDPTE ATAVGAVEAS FKCCSGAIIV LTKSGRSAHQ VARYRPRAPI IAVTRNPQTA RQAHLYRGIF

Predicted Isoelectric Point:
8.3

Predicted Molecular Mass:
49.3kD

Accurate Molecular Mass:
50kD as determined by SDS-PAGE reducing conditions.

Subcellular Location:
Nucleus, Cytoplasm

Applications:
Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -70°C. Stable for 6 months after receipt. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Note:
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.