Protease Inhibitor Cocktail, Set II

Molecular Biology / MB-Protease Inhibitors

-20°C

Supplied as a lyophilized powder.

A cocktail of five protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, serine, and metalloproteases as well as aminopeptidases. This cocktail is recommended for use with bacterial cell extracts.

Note: 1 set = 1 vial of lyophilized protease inhibitor cocktail and 1 vial DMSO, 1ml; 5 sets = 5 vials of lyophilized protease inhibitor cocktail and 5 vials of DMSO, 1ml.

Kit Components:
AEBSF, Hydrochloride: Molecular weight: 239.5, 1X20mM. Target Protease: Serine Proteases
Bestatin: Molecular weight: 308.4, 1X1.7mM. Target Protease: Aminopeptidase B and Leucein Aminopeptidase
E-64 Protease Inhibitor: Molecular weight: 357.4, 1X200uM. Target Protease: Cysteine Proteases
EDTA, Tetrasodium: Molecular weight: 416.2, 1X85mM. Target Protease: Metalloproteases
Pepstatin A: Molecular weight: 685.9, 1X2mM. Target Protease: Aspartic Proteases

Solubility:
DMSO:H2O (1:4)

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.