Protease Inhibitor Cocktail, Set III, Animal-Free

Molecular Biology / MB-Protease Inhibitors

-20°C

Supplied as a liquid in 1ml DMSO.

A cocktail of six protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases. This cocktail is recommended for use with mammalian cell and tissue extracts. Each vial contains 100mM AEBSF, HCl, 80uM Aprotinin, 5mM Bestatin, 1.5mM E-64, 2mM Leupeptin Hemisulfate and 1mM Pepstatin A. Available as 1ml vial or a set of 5x1ml.

Kit Components:
AEBSF, Hydrochloride: Molecular weight: 239.5, 1X100mM. Target Protease: Serine Proteases
Aprotinin, Recombinant, Bovine, Nicotiana sp., Animal-free: 1X80uM. Target Protease: Broad Sprectrum, Serine Proteases
Bestatin: Molecular weight: 308.4, 1X5mM. Target Protease: Aminopeptidase B and Leucein Aminopeptidase
E-64 Protease Inhibitor: Molecular weight: 357.4, 1X1.5mM. Target Protease: Cysteine Proteases
Leupeptin, Hemisulfate: Molecular weight: 475.6, 1X2mM. Target Protease: Cysteine Proteases and Trypsin-like Proteases
Pepstatin A: Molecular weight: 685.9, 1X1mM. Target Protease: Aspartic Proteases

Form:
Supplied as a liquid in 1ml DMSO.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.