Protein A (HRP) (Lyophilized)

Molecular Biology / MB-BioSep Protein A/G/L

-20°C

Supplied as a lyophilized powder from 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 10mg/ml BSA, and 0.01% gentamicin sulfate. Labeled with HRP. Rehydration: Add 500ul sterile, distilled water to make a 2mg/ml stock solution.

Protein A (from Staphylococcus aureus, Cowan I Strain) is conjugated with highly purified horseradish peroxidase (RZ > 3.0).

Applications:
Suitable for use in Immunoblot (Western or Dot Blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:2000-1:10, 000
Dot Blot: 1:2000-1:10, 000
ELISA: 1:20, 000-1:100, 000
Immunohistochemistry: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder is stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.