Protein G, Native (HRP)

Molecular Biology / MB-BioSep Protein A/G/L

-20°C

Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% Gentamicin sulfate. Labeled with Horseradish peroxidase (HRP).Reconstitute with 1ml sterile ddH2O.

Native Protein G is a surface receptor from Streptococcus sp. which binds the Fc portion of immunoglobulins, binding 2 molecules of IgG.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:20, 000-1:200, 000
Western Blot: 1:10, 000-1:40, 000
Immunohistochemistry: 1:1000-1:5000
Optimal dilution determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.