11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) BioAssay™ ELISA Kit (Human)

Kits and Assays / Kits-ELISA, BioAssay™

4°C/-20°C

11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) BioAssay™ ELISA Kit (Human) is a sandwich enzyme immunoassay for the in vitro quantitative measurement of HSD11b1 in human tissue homogenates, cell Lysates and other biological fluids.

Detection Range:
1.56-100ng/ml

Sensitivity:
≤0.57ng/ml

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components:
*023015A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use
*023015B: Standard, 2x1 vial
023015C: Standard Diluent, 1x20ml
*023015D: Detection Reagent A, 1x120ul
*023015E: Detection Reagent B, 1x120ul
023015F: Assay Diluent A, 1x, 1x12ml
023015G: Assay Diluent B, 1x, 1x12ml
023015H: TMB Substrate, 1x9ml
023015K: Stop Solution, 1x6ml
023015L: Wash Buffer, 30X, 1x20ml

Precaution:
The Stop Solution (023015K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability:
Store *023015A, *023015B, *023015D and *023015E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.